Table 1 Percentage of mutated plants in the T0 generation induced by single-gene targeted sgRNAs.
Target Gene | sgRNA | No. of Plants examined | No. of plants with mutations | Mutation rate (%) | Homozygous mutations | |
|---|---|---|---|---|---|---|
Number | Rate (%) | |||||
BnaA9.RGA | sgRNA1 | 30 | 24 | 80.0 | 0 | 0 |
sgRNA2 | 21 | 70.0 | 0 | 0 | ||
BnaC9.RGA | sgRNA1 | 29 | 29 | 100.0 | 0 | 0 |
sgRNA2 | 29 | 100.0 | 0 | 0 | ||
BnaA6.RGA | sgRNA1 | 30 | 19 | 63.3 | 0 | 0 |
sgRNA2 | 21 | 70.0 | 0 | 0 | ||
BnaC7.RGA | sgRNA1 | 19 | 1 | 5.3 | 0 | 0 |
sgRNA2 | 8 | 42.1 | 0 | 0 | ||
BnaA2.DA2 | sgRNA1 | 41 | 37 | 90.2 | 1 | 2.4 |
sgRNA2 | 18 | 43.9 | 0 | 0 | ||
BnaA6.DA1 | sgRNA1 | 64 | 41 | 64.1 | 0 | 0 |
sgRNA2 | 26 | 40.6 | 4 | 6.25 | ||
BnaC5.DA1 | sgRNA1 | 20 | 8 | 40.0 | 0 | 0 |
sgRNA2 | 20 | 100.0 | 0 | 0 | ||
Total (average) | Â | 233 | Â | (65.3) | 5 | (0.6) |
