Table 2 Percentage of mutated sites in the T0 generation induced by multiple-gene targeted sgRNAs.

From: CRISPR/Cas9-mediated genome editing efficiently creates specific mutations at multiple loci using one sgRNA in Brassica napus

Gene family

Target Gene

sgRNA

No. of Plants examined

No. of plants with mutations

Mutation rate (%)

Homozygous mutations

% of homozygous mutations

% of plants mutated at different target genes

BnaRGA

BnaA9.RGA

sgRNA1

29

28

96.6

3

10.3

One gene is mutated: 0 Two genes are mutated: 3.4 Three genes are mutated: 6.9 Four genes are mutated: 86.2

sgRNA2

8

27.6

0

0

BnaC9.RGA

sgRNA1

29

25

86.2

0

0

sgRNA2

9

31

0

0

BnaA6.RGA

sgRNA1

29

28

96.6

6

20.7

sgRNA2

10

34.5

0

0

BnaC7.RGA

sgRNA1

29

27

93.1

5

17.2

sgRNA2

12

41.4

0

0

BnaDA2

BnaA2.DA2

sgRNA1

17

7

41.2

0

0

One gene is mutated: 35.3 Two genes are mutated: 47.1

sgRNA2

8

47.1

1

5.9

BnaC6.DA2

sgRNA1

17

6

35.3

0

0

sgRNA2

14

82.4

1

5.9

BnaFUL

BnaA9.FUL

sgRNA1

21

16

76.2

0

0

One gene is mutated: 0 Two genes are mutated: 22.2 Three genes are mutated: 55.6

sgRNA2

18

85.7

4

19

BnaC2.FUL

sgRNA1

21

16

76.2

0

0

sgRNA2

17

81

4

19

BnaC7.FUL

sgRNA1

21

16

76.2

0

0

sgRNA2

18

85.7

3

14.3

Total

  

67

    
Back to article page