Table 3 Genotypes of T0 transgenic plants. ‘—’: the sequencing results were not well decoded by DSDecode.
Target gene | Sites | No. of examined plants | Genotype | ||||
|---|---|---|---|---|---|---|---|
Homozygote | Heterozygote | Bi-allele | Chimera | WT | |||
BnaA9.RGA | BnaA9.RGA-sgRNA1 | 20 | 8 (40.0%) | 1 (5.0%) | 6 (30%) | 5 (25.0%) | |
BnaA9.RGA-sgRNA2 | — | ||||||
BnaA9.RGA | BnaRGA-sgRNA1 | 23 | 3 (13.0%) | 1 (4.3%) | 13 (56.5%) | 5 (21.8%) | 1 (4.3%) |
BnaRGA-sgRNA2 | 8 | 5 (62.5%) | 2 (25.0%) | 1 (12.5%) | |||
BnaC9.RGA | BnaC9.RGA-sgRNA1 | 4 | 4 (100.0%) | ||||
BnaC9.RGA-sgRNA2 | 5 | 5 (100.0%) | |||||
BnaC9.RGA | BnaRGA-sgRNA1 | 11 | 1 (9.1%) | 4 (36.4%) | 5 (45.5%) | 1 (9.1%) | |
BnaRGA-sgRNA2 | 4 | 2 (50.0%) | 1 (25.0%) | 1 (25.0%) | |||
BnaA6.RGA | BnaA6.RGA-sgRNA1 | 25 | 4 (16.0%) | 3 (12.0%) | 10 (40.0%) | 8 (32.0%) | |
BnaA6.RGA-sgRNA2 | 4 | 3 (75.0%) | 1 (25.0%) | ||||
BnaA6.RGA | BnaRGA-sgRNA1 | 29 | 6 (20.7%) | 2 (6.9%) | 14 (48.3%) | 6 (20.7%) | 1 (3.4%) |
BnaRGA-sgRNA2 | 8 | 1 (12.5%) | 1 (12.5%) | 5 (62.5%) | 1 (12.5%) | ||
BnaC7.RGA | BnaC7.RGA-sgRNA1 | 19 | 1 (5.3%) | 18 (94.7%) | |||
BnaC7.RGA-sgRNA2 | 19 | 3 (15.8%) | 1 (5.3%) | 4 (21.1%) | 11 (57.9%) | ||
BnaC7.RGA | BnaRGA-sgRNA1 | 30 | 5 (16.7%) | 3 (10.0%) | 16 (53.3%) | 5 (16.7%) | 1 (3.3%) |
BnaRGA-sgRNA2 | 11 | 8 (72.7%) | 3 (27.3%) | ||||
BnaC5.DA1 | BnaC5.DA1-sgRNA1 | 20 | 3 (15.0%) | 3 (15.0%) | 4 (20.0%) | 10 (50.0%) | |
BnaC5.DA1-sgRNA2 | 17 | 17 (100.0%) | |||||
BnaA6.DA1 | BnaA6.DA1-sgRNA1 | — | |||||
BnaA6.DA1-sgRNA2 | 29 | 4 (13.8%) | 4 (13.8%) | 8 (27.6%) | 5 (17.2%) | 8 (27.6%) | |
BnaA2.DA2 | BnaDA2-sgRNA1 | — | |||||
BnaDA2-sgRNA2 | 17 | 1 (5.9%) | 5 (29.4%) | 1 (5.9%) | 2 (11.8%) | 8 (47.1%) | |
BnaC6.DA2 | BnaDA2-sgRNA1 | 16 | 1 (6.25%) | 1 (6.25%) | 7 (43.75%) | 7 (43.75%) | |
BnaDA2-sgRNA2 | 16 | 1 (6.25%) | 3 (18.75%) | 2 (12.5%) | 8 (50.0%) | 2 (12.5%) | |
BnaC2.FUL | BnaFUL-sgRNA1 | 15 | 1 (6.7%) | 1 (6.7%) | 9 (60.0%) | 4 (26.6%) | |
BnaFUL-sgRNA2 | 16 | 4 (25.0%) | 3 (18.75%) | 5 (31.25%) | 2 (12.5%) | 2 (12.5%) | |
BnaA9.FUL | BnaFUL-sgRNA1 | 19 | 1 (5.3%) | 1 (5.3%) | 13 (68.4%) | 4 (21.0%) | |
BnaFUL-sgRNA2 | 19 | 4 (21.1%) | 1 (5.3%) | 5 (26.3%) | 7 (36.8%) | 2 (10.5%) | |
BnaC7.FUL | BnaFUL-sgRNA1 | 17 | 13 (76.5%) | 4 (23.5%) | |||
BnaFUL-sgRNA2 | 18 | 3 (16.7%) | 1 (5.6%) | 7 (38.9%) | 3 (16.7%) | 4 (22.2%) | |
Total | 439 | 34 (7.7%) | 71 (16.1%) | 90 (20.5%) | 140 (31.9%) | 104 (23.7%) | |
