Table 5 Detection of mutations at the putative CRISPR/Cas9 off-target sites in the T0 and T1 generation.
Target | Putative off-target sites | Putative off-target locus | Putative off-target sequence | No. of mismatch bases | No. of plants examined | No. of mutations |
|---|---|---|---|---|---|---|
BnaA9.RGA-sgRNA1 | OFF1 | chrC09_random:+1591592 | GAGGTCGTCAGAGATGGCGGAGG | 2 | 50 | 0 |
OFF2 | chrC07:-27532718 | CAGGTCGTCGGAGATGGCTGAGG | 3 | 50 | 0 | |
OFF3 | chrC09_random:+1591705 | ACCCGTCGGAGCTTTACTCGTGG | 1 | 50 | 0 | |
BnaC9.RGA-sgRNA1 | OFF1 | chrA09:−11644254 | CAAGGTGAGGTCGTCCGAGATGG | 1 | 50 | 0 |
OFF2 | chrA09:−11644135 | ACCCGTCGGAGCTCTACTCGTGG | 1 | 50 | 0 | |
OFF3 | chrA06:+23009274 | ACCCCGCTGAGCTTTACTCGTGG | 3 | 50 | 0 | |
BnaA6.RGA-sgRNA1 | OFF1 | chrC09:+5730399 | CAAGGTAAGGTCGTCGGAGATGG | 2 | 50 | 0 |
OFF2 | chrC07:−33809295 | CAAGGTAAGGTCGTCGGAGATGG | 2 | 50 | 0 | |
OFF3 | chrC07:−27532724 | CAAGGTCAGGTCGTCGGAGATGG | 2 | 50 | 0 | |
OFF4 | chrC09_random:+1591705 | ACCCGTCGGAGCTTTACTCGTGG | 3 | 50 | 0 | |
BnaC7.RGA-sgRNA1 | OFF1 | chrC07:−33809289 | AAGGTCGTCGGAGATGGCGGAGG | 2 | 50 | 0 |
OFF2 | chrC09:+5730405 | AAGGTCGTCGGAGATGGCGGAGG | 2 | 50 | 0 | |
OFF3 | chrA06:+23009161 | TAGGTCTTCGGAGATGGCTGAGG | 2 | 50 | 0 |
