Figure 5
MiR-33b significantly decreased mast cell degranulation and PCA reactions. (A) BMMCs from C57BL/6 mice were sensitized with 1 μg/ml OVA IgE (E-C1) for 24 h and then stimulated with 10 μg/ml OVA for 30 min. Degranulation was monitored as the release of β-hexosaminidase. (B,C) miR-33b decreased the IgE-mediated LTC4 and IL-13 production. (D) Representative images of Evans blue-stained extravasation into the paws 30 min after intravenous injection of OVA in C57BL/6 mice treated with miR-NC BMMCs or miR-33b BMMCs (n = 6 each group). (E) Quantification of the extravasation of Evans blue leakage into the paws. The absorbance was measured at 620 nm, and the data are expressed as Evan’s blue in ng/mg tissue. The data are presented as the means ± SEM. Comparisons between miR-NC BMMC- and miR-33b BMMC-transfected C57BL/6 mice were performed using two-tailed Student’s t-test. *P < 0.05.
