Figure 3 | Scientific Reports

Figure 3

From: Blockage of the lysosome-dependent autophagic pathway contributes to complement membrane attack complex-induced podocyte injury in idiopathic membranous nephropathy

Figure 3

Assembly of sublytic C5b-9 on podocytes after exposure to zymosan activation serum (ZAS). (A,B) Lactate dehydrogenase (LDH) assay, quantified by the optical density of the supernatant at 450 nm. The release rate of LDH from ZAS-treated podocytes gradually increased in a volume fraction-dependent manner as of the 1-h time point. After exposure of podocytes to ZAS at 10% dilution, the release rate of LDH increased by less than 10% within 12 h, and then further increased at 24 h to 36 h. The data represent mean ± SD. (C) Immunofluorescent showing a lot of C5b-9-positive puncta in ZAS (10%)-treated podocytes from 1 h to 36 h, while rare in HIS (10%)-treated podocytes. The nuclei were stained with DAPI (blue). Images were obtained using a confocal microscope. Scale bar, 25 μm.

Back to article page