Figure 5
From: Double-stranded RNA induces chicken T-cell lymphoma apoptosis by TRIF and NF-κB
Poly(I:C)-induced apoptosis mediated by NF-κB activation, independently of type I IFN signaling. (A) Verification of IRF3 inhibitor effect in avian cells. (B) Verification of NF-κB inhibitor effect in avian cells. (C) MDCC-MSB1 cells were pre-treated with the BX795, resveratrol, BAY11-7089, or DMSO (used as control) before culture with or without poly (I:C) (10 μg/ml) for 24 hours. The percentage of cell viability is shown. (D) The percentage of apoptotic cells in MDCC-MSB1 cells, treated as described in C, was shown. (E) The caspase activity of MDCC-MSB1 cells, obtained as described in D, was measured. (F) Transcriptional kinetic of pro-apoptotic factors in MDCC-MSB1 cells, treated as described in C, was shown. The asterisk (*) or double asterisk (**) respectively indicates p < 0.05 or p < 0.01 in statistical difference from controls. The bars represent an average of multiple experiments.
