Figure 5 | Scientific Reports

Figure 5

From: Dynamic Mechano-Regulation of Myoblast Cells on Supramolecular Hydrogels Cross-Linked by Reversible Host-Guest Interactions

Figure 5

In situ mechano-response of C2C12. Dynamic response of C2C12 myoblasts upon the softening of βCD–Ad gel (2,2) from E ≈ 11 to 4 kPa, caused by the exchange to the medium containing 10 mM of βCD. (a) Snapshot images of a C2C12 cell from the live cell imaging (LifeAct-GFP). (b) Actin cytoskeletons extracted from the images. Changes in (c) projected cell area A, (d) aspect ratio AR, (e) circularity C, and (f) nematic order parameter of actin cytoskeleton <S> are plotted over time. The medium was exchanged at t = 50 min, and medium containing βCD was continuously supplied in the shaded time zone.

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