Figure 7
Effect of Hyp-GQDGLAGPK on inflammatory markers in the lacrimal glands of experimental dry eye mouse model. (A) The sections were immunostained with specific antibodies for TNF-α, ICAM-1, VCAM-1, and MMP2 before desiccation stress, immediately after desiccation stress for 10 days, and 10 days after treatment with Hyp-GQDGLAGPK, cyclosporine, diquafosol, and sodium hyaluronate eye drops. Scale bar = 100 μm. (B) The stained area of the photograph was analyzed using ImageJ® and calculated in terms of the fold of the control. Values are mean ± SD (n = 8~10 eyes). The results are statistically significant by analysis of variance with the Tukey test at *p < 0.05 compared with baseline, # p < 0.05 compared with DS 10d and † p < 0.05 vs corresponding value in the normal saline group. Con = control; DS 10d = immediately after desiccation stress for 10 days; NS = normal saline; 10mer = Hyp-GQDGLAGPK; Cys = cyclosporine; Diq = diquafosol; HA = sodium hyaluronate; TNF-α = tumor necrosis factor alpha; ICAM-1 = intercellular adhesion molecule-1; VCAM-1 = vascular cell adhesion molecule-1; MMP-2 = matrix metalloproteinase 2.
