Figure 6 | Scientific Reports

Figure 6

From: Genetic variation at the CD28 locus and its impact on expansion of pro-inflammatory CD28 negative T cells in healthy individuals

Figure 6

GG, AA and AG individuals show similar frequencies of Tregs. CD4+CD25− T cells were activated in vitro with aCD3 antibody and CHO-CD80 cell line in the presence or absence of 1,25(OH)2D3 for 5 days. (A) Representative flow cytometry plots showing expression of Foxp3 and CTLA4 on CD25+ cells across genotypes in the presence (VitD+) and absence (VitD−) of 1,25(OH)2D3. Values in cross symbols represent the mean frequency (%) of Foxp3hi CTLA4+ and Foxp3int CTLA4+ plus/minus standard deviation across genotypes in all samples (n = 14 per group). (B) Frequency of Foxp3hi CTLA4+ in CD25+ cells in the presence and absence of vitamin D. (C,D) Foxp3 and CTLA4 median fluorescence intensity (MFI) on Foxp3hi CTLA4+ cells.

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