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Figure 1

From: The [PSI +] yeast prion does not wildly affect proteome composition whereas selective pressure exerted on [PSI +] cells can promote aneuploidy

Figure 1

Establishment of the [psi −] and [PSI +] strains for SILAC analysis. (a) Schematic diagram of the SILAC approach. [PSI +] cells were generated following the galactose-induced overexpression of exogenous Sup35NM, and selection of ade1-14 cells in media lacking adenine. (b) Indicated strains were grown in the indicated plates. (c) SDD-AGE of the lysates of the indicated strains with anti-Sup35 antibodies. (d) Colonies from [PSI +]SILAC and [psi −]SILAC cells that were grown for 5 days on 1/4 YPD plates (see also Fig. S1a).

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