Figure 4 | Scientific Reports

Figure 4

From: The [PSI +] yeast prion does not wildly affect proteome composition whereas selective pressure exerted on [PSI +] cells can promote aneuploidy

Figure 4

Chromosome instability (CIN). (a) The indicated strains transformed with pRS316 were plated on YPD to allow the formation of colonies for 2 days. The colonies were then replicated on SD+Arg−Ura plates. The percentage loss of the URA3-containing plasmid was calculated by comparing the number of colonies on each plate: (YPD − SD)/YPD. The standard deviations were calculated from 5 replicates. (b) The percentage of pRS316 loss was calculated as in a, but after the incubation of [psi −]SILAC cells transformed with the indicated HIS3 plasmids in YP-raffinose media with or without galactose for 48 hours. (c,d) Relative quantity of DNA from chromosome I left and right arm compared to [psi −], using qPCR. Genomic DNA was extracted from [PSI +] #1 samples grown in YPD before (t = 0hr; YPD) and after curing in 4 mM guanidine hydrochloride (GdnHCl) (t = 36 hr; YPD + GdnHCl). [psi −] was used as a control, as well as [PSI +]#1 grown for 36 hrs in YPD alone (t = 36 hr; YPD). Strains were verified to be cured by plating on 1/4 YPD (no GdnHCl) and SD−Ade+Arg (c). Gene regions from chromosome 1 left and right arms were done in triplicates, while error bars represent standard deviation (d).

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