Figure 6
Survey of chromosome copy number by whole genome sequencing. (a,b) For sequencing reads matching each indicated chromosome, the log2 ratios of the “normalized reads” (i.e., percent of reads matching a given chromosome in a given strain) divided by the “median normalized reads” (i.e., median of percentages of reads matching a given chromosome in all [psi −] strains) were reported for 30 [psi −]SILAC strains (a) and 30 [PSI +]SILAC strains (b) selected on YPD plates after Sup35NM overexpression. Each strain is represented by a line in a different blue colour. (c) mtDNA levels of the indicated strains were quantified by comparing relative mitochondrial COX1 vs. nuclear ACT1 genes using qPCR. The genomic DNA (gDNA) used for the NGS analysis was analyzed (grey), as well as gDNA obtained from the same strains that were kept in the logarithmic growth phase (blue). Standard deviations are from three technical replicates. #” designates strains that were generated for the whole genome analysis. (d) 12 [psi −] and [PSI +] strains previously used for NGS were grown for 24 hours in YPD with excess adenine (100 mg/mL) to stationary phase. mtDNA levels were then quantified by comparing relative mitochondrial COX1 vs. nuclear ACT1 genes using qPCR.
