Figure 4
From: Secretory phospholipase A2 modified HDL rapidly and potently suppresses platelet activation
HDL-associated LPCs mediate sPLA2-HDL effects on platelets. (a) Washed platelets were preincubated with vehicle or HDL enriched with different LPCs (16:0, 18:0, 18:1, 18:2, 20:4) or FFAs (16:0, 18:1). Subsequently, platelets were stimulated with ADP inducing 70–90% aggregation in vehicle-stimulated platelets. (b) Platelets were stimulated with vehicle, HDL enriched with FFA 18:2, HDL enriched with FFA 20:4 or with ADP (20 µM) as a positive control and the aggregation was recorded. Values are expressed as % of maximal platelet aggregation. Results are shown as mean ± SEM (n = 3). Statistical significance was assessed by one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001 versus vehicle.Results shown are mean ± SEM (n = 3).
