Figure 2 | Scientific Reports

Figure 2

From: TRPM6 and TRPM7 differentially contribute to the relief of heteromeric TRPM6/7 channels from inhibition by cytosolic Mg2+ and Mg·ATP

Figure 2

Assessment of mTRPM6 and mTRPM7 currents in HEK 293 and TS cells. (A) Whole-cell currents measured in mTRPM6 (red), mTRPM7-transfected (blue) and untransfected (black) HEK 293 cells. Current amplitudes (mean ± SEM) were acquired at −80 and +80 mV and plotted over time. (B) Magnification of mTRPM6 and endogenous TRPM7 currents shown in (A). (C) Representative current-voltage (I-V) relationships of currents (at 90 s) illustrated in (A). (D) Bar graphs of outward (Upper panel) and inward (Lower panel) currents (mean ± SEM) shown in (A) at 90 s. n, number of cells measured; n.s., not significant; **P < 0.01; ***P < 0.001 (ANOVA). (E,F) Functional expression of mTRPM6 in trophoblast stem (TS) cells. Trpm7-gene deficient TS cells were electroporated with mTRPM6 cDNA and examined as described in (A). (E) Whole-cell currents (mean ± SEM) measured in mTRPM6-transfected (red) and untransfected (black) TS cells. (F) I-V relationships of the currents (at 90 s) shown in (E). n, number of cells measured.

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