Figure 3 | Scientific Reports

Figure 3

From: TRPM6 and TRPM7 differentially contribute to the relief of heteromeric TRPM6/7 channels from inhibition by cytosolic Mg2+ and Mg·ATP

Figure 3

Assessment of mTRPM6 and mTRPM7 currents in a divalent cation-free (DVF) extracellular solution. Whole-cell currents measured in mTRPM7-transfected (A), untransfected (B) and mTRPM6-transfected HEK 293 cells (C). Left panels: Current amplitudes (mean ± SEM) were measured at −80 and +80 mV and plotted over time. Currents were induced using the standard [Mg2+]i-free intracellular solution and the standard external solution. When currents were fully activated, cells were perfused with the DVF solution as indicated by the black bars. Right panels: Representative I-V relationships obtained from individual ramps before (blue), during (red and green) and after (violet) DVF application as indicated in the Left panels by coloured data points. n, number of cells measured.

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