Figure 5
Determination of divalent cations permeability of mTRPM6. Measurements were performed similarly to Fig. 4. (A) Left panel: Whole-cell currents were recorded in mTRPM6-transfected HEK 293 cells using the standard [Mg2+]i-free internal solution and standard external saline. When currents started to develop, the cells were exposed to an external solution containing 10 mM Zn2+ as indicated by a bar. Data are shown as I/Imax ± SEM (Imax value was obtained in a ramp before application of 10 mM Zn2+). Middle panel: Representative I-V relationships of inward currents obtained before (blue) and during (red) application of 10 mM Zn2+ as indicated in the Left panel. Right panel: Bar graphs of inward currents (−80 mV, mean I/Imax ± SEM) obtained before (blue) and during (red) application of 10 mM Zn2+ as indicated in the Left panels. (B,C) Changes in the inward mTRPM6 currents by exposure of cells to external solutions containing either 10 mM Mg2+ (B) or 10 mM Ca2+ (C). n, number of cells measured; n.s., not significant; **P < 0.01; ***P < 0.001 (two-tailed t-test).
