Figure 7
Effects of cytosolic Mg·ATP on mTRPM6 and mTRPM7 currents. (A) Whole-cell currents (mean ± SEM) measured in mTRPM7-transfected HEK 293 cells (A), untransfected cells (B), or cells transfected either by wildtype mTRPM6 (C) or mTRPM6-K1810R variant (D). Cells were perfused either with the standard [Mg2+]i-free intracellular solution or with a solution containing 210 µM Mg·ATP (10 µM free Mg2+). (E) Bar graphs of outward currents (−80 mV) shown in (A–D). Current amplitudes (mean ± SEM) were extracted at time intervals when the currents were maximally induced by the Mg2+-free intracellular solution as follows: at 200 s for mTRPM7 (blue) and untransfected cells (black), 50 s for wildtype mTRPM6 (red) and mTRPM6-K1810R (green). n, number of cells measured; n.s., not significant; **P < 0.01; ***P < 0.001 (two-tailed t-test).
