Figure 3

Isoflurane anesthesia regulates intracellular signaling implicated in rapid antidepressant actions. (A) Phosphorylation of Akt^T308 (p = 0.0084), CREB^S133 (p = 0.0087), mTOR^S2481 (p = 0.0292), p70S6K^T421/S424 (p = 0.0022), 4-EBP1^T37/46 (p = 0.0012) and GSK3β^S9 (p = 0.0001) in the adult mouse prefrontal cortex after isoflurane anesthesia (30 min). (B) Phosphorylation of Akt^T308 (p = 0.3052), CREB^S133 (p = 0.0014), mTOR^S2481 (p = 0.7422), p70S6K^T421/S424 (p = 0.0022) 4E-BP1^T37/46 (p = 0.0022) and GSK3β^S9 (p = 0.0001) in the adult mouse hippocampus after isoflurane anesthesia (30 min). (C) Phosphorylation of CREB^S133, mTOR^S2481 and p70S6K^T421/S424 are increased within 2 minutes from the onset of isoflurane (4%) administration. pAKT and pCREB levels normalized to corresponding total protein, other phosphoproteins normalized to GAPDH. n = 6/group. Representative western blots have been cropped from complete immunoblots shown in Supplementary Information file. *p < 0.05, **p < 0.01, ***p < 0.001; Student’s t test. Abbreviations: CTRL, control treatment; ISO, isoflurane treatment; CREB, cAMP response element binding protein; Akt, protein kinase B; mTOR, mammalian target of rapamycin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase, GSK3β, glycogen synthase kinase 3β.