Figure 5
From: Isoflurane produces antidepressant effects and induces TrkB signaling in rodents
Lack of effects of isoflurane anesthesia on dendritic spines in the mouse cortex and hippocampus. Reconstruction of confocal images showing the medial prefrontal cortex (mPFC) (A and E), the hippocampus (HC) (F) and somatosensory cortex (SSCx) (G) from Thy1-eYFP mice. We analyzed dendritic spine density of the primary apical dendrites of pyramidal neurons in three different segments of 60 µm each up to 180 µm from the cell soma (B; also indicated with *). Representative images of distal dendritic segment between 120 and 180 µm (C). We also analyzed extra-distal dendritic segments in the mPFC and the HC (D; indicated as # in E and F). We analyzed dendritic spine densities from mice subjected to sham or isoflurane treatment (30 min) 24-h before. Histograms showing the densities of spine subtypes in three different segments relative to the distance to the soma (B) in the mPFC (H; indicated as *), HC (I; *) and the SSCx (J; *). Isoflurane treatment did not produce changes to the spine morphology or to the total spine amount at different distances from the soma (2-way ANOVA: treatment effect for distance in the mPFC (F1,36 = 0.7705, p = 0.385), the HC (F1,21 = 1.371, p = 0.2547) and the SSCx (F1,36 = 0.02270, p = 0.8688)). Extra distal segments were also analyzed in the mPFC (H; indicated as #p = 0.6756) and the HC (I; #p = 0.8203). n = 7 mice/group, 3 sections/mouse, 8 dendrites/section. Scale bar in A and D: 4 μm. Statistical analysis was done using Student’s t test (for extra distal segments or # in H and I) and 2-way ANOVA (for the first 180 µm from the cell soma or * in H,I and J).
