Figure 2

Primary mouse ATI-like cells express COX-2 and COX-1 and A549 cells only COX-2. (a) A549 or (b,c) mouse primary ATI-like cells were treated with vehicle (Veh), LPS (10 µg/ml) and/or dexamethasone (dexa; 1 µM) and (a,b) COX-2 or (c) COX-1 expression was determined by Western blot. A549 cells did not express COX-1. Densitometric values normalized to beta actin are shown as mean + SEM. Inserts on the right show sections of typical blots (lanes vehicle: 1,5,9; LPS: 2,6,10; dexamethasone: 3,7,11; LPS + dexamethasone: 4,8,12). The inserts in (b) and (c) show the same membrane and thus the same loading control was used. n = 6 for A549 cells and n = 5 for primary ATI-like cells. Statistical analysis was performed using One Way ANOVA and Bonferroni’s post-hoc test, *p < 0.05; **p < 0.01; ***p < 0.001.