Figure 7
From: The Role of PGE2 in Alveolar Epithelial and Lung Microvascular Endothelial Crosstalk
Conditioned medium protects endothelial cells from thrombin-induced disruption of adherens junctions and stress fiber formation. HMVEC-L were grown in chamber slides and pretreated with either vehicle, the EP4 antagonist ONO AE3 208 (EP4-Ant; 300 nM) or the S1P1 antagonist W146 (S1P1-ANT; 10 µM) as indicated. Conditioned medium (CM) from (a,b) ATI-like cells or (c,d) A549 cells was added followed by 0.5 U/ml of thrombin or vehicle for 15 min. Afterwards, cells were stained with (a,c) an antibody against VE-cadherin (green) to visualize adherens junctions and (b,d) phalloidin (red) for stress fiber formation. Nuclei were counterstained using DAPI. Images are representative for 5 different experiments.
