Table 1 Antibodies (AB) used in Western blots (WB) and immunofluorescence (IF) microscopy.
From: The Role of PGE2 in Alveolar Epithelial and Lung Microvascular Endothelial Crosstalk
antibody | host species | company | number | IF dilution | WB dilution | |
|---|---|---|---|---|---|---|
primary AB | COX2 | rabbit | Abcam | 15191 | 1:500 | 1:1000 |
COX1 | goat | Santa Cruz | sc-1754 | 1:250 | 1:200 | |
pro-SP-C | rabbit | Seven Hills | WRAB-9337 | 1:1000 | — | |
AQP5 | rabbit | Alomone Labs | AQP-005 | 1:500 | — | |
beta-actin | mouse | Sigma | A2228 | — | 1:7500 | |
VE-cadherin | mouse | Santa Cruz | sc-9989 | 1:200 | — | |
EP4 receptor | rabbit | Abcam | 133170 | — | 1:200 | |
F’ab fragments | goat | Jackson Immunoresearch | 111-007-003 | 1:50 | — | |
secondary AB | anti rabbit Cy3 | goat | Invitrogen | A-10520 | 1:500 | — |
anti goat AF488 | rabbit | Invitrogen | A-11078 | 1:500 | — | |
anti rabbit AF488 | goat | Invitrogen | A-11008 | 1:500 | — | |
anti goat AF594 | donkey | Invitrogen | A-11058 | 1:250 | — | |
anti rabbit HRP | goat | Cell signaling | 70745 | — | 1:1000 | |
anti goat HRP | donkey | Jackson Immunoresearch | 705-035-003 | — | 1:1000 | |
anti mouse HRP | goat | Jackson Immunoresearch | 115-036-062 | — | 1:1000 |
