Figure 4

DACT1 suppresses Wnt/β-catenin signalling by inducing Dvl2 degradation and β-catenin nuclear translocation. (a) and (b) The Dvl2 and β-catenin content of 3AO cells was significantly lower in cells overexpression DACT1. Experiments were performed in triplicate and grey values were measured by Fusion (*P < 0.05, ****P < 0.0001). (NC: negative control; WT: wild type). (c) and (d) Overexpression of DACT1 also significantly reduced cellular levels of GSK-3β with phosphorylated Ser9, and the Wnt/β-catenin target genes, CyclinD1 and C-myc. Grey values collected by Fusion were analysed (*P < 0.05, ***P < 0.001). (e) and (f) Nuclear protein was extracted and demonstrated that β-catenin translocation to the nucleus was significantly decreased by overexpression of DACT1. α-tubulin was used as the loading control. Grey values collected by Fusion were analysed. (****P < 0.0001). All the experiment was repeated at least three times.