Figure 3 | Scientific Reports

Figure 3

From: RNA sequencing of db/db mice liver identifies lncRNA H19 as a key regulator of gluconeogenesis and hepatic glucose output

Figure 3

H19 levels are downregulated in the db/db mice liver. (a) 1 µg of total RNA was reverse transcribed and expression levels of H19 lncRNA in the livers of db/+ (n = 8) and db/db (n = 10) mice were quantified by qRT-PCR using specific primers. β-actin was used as a normalization control. (b) Expression levels of hepatic Igf2 was quantified in db/+ (n = 10) and db/db (n = 10) mice by qRT-PCR using specific primers. (c) 1 µg of total RNA was reverse transcribed using specific stem loop primers for miR-675 and its expression was quantified in the livers of db/+ (n = 8) and db/db (n = 10) mice. U6 was used as the normalization control. Data presented are means ± s.e.m. *P < 0.05.

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