Figure 6

Partial inhibition of the effect of malaria in the fetus development of pregnant C57BL/6 mice infected with P. berghei NK65^GFP by using IAXO-101 as TLR4 antagonist. Fetuses weight from pregnant WT mice infected and treated with IAXO-101 (A). Placental vascular space (B) obtained at G19 from pregnant WT mice: non-infected (black open circle), infected (black filled circle), infected treated with IAXO-101 (blue circle), and infected treated with IAXO-101 vehicle (pink square circle). Serum levels of TNF-α measured by cytometric bead arrays (CBA) (C), and parasitemia (D) of pregnant WT mice infected and treated with IAXO-101 (A). As a control, infected pregnant mice were treated with the IAXO-101 vehicle. Mice were infected with 1 × 10⁵ iRBCs i.v. on G13. IAXO-101 treatment was conducted at the same day of the infection with 5 mg/Kg/day by six consecutive days. The animals were euthanized on the 6^th day post-treatment. The maternal blood space areas on labyrinth layer were quantified in relation to the total area of the placenta by using an automated morphometric analysis method. Data are presented as scatter plot with an indication of the median. One-way ANOVA: *P-value < 0.05; **P-value < 0.01; ***P-value < 0.001.