Figure 3 | Scientific Reports

Figure 3

From: 2,4,6-Trinitrotoluene Induces Apoptosis via ROS-Regulated Mitochondrial Dysfunction and Endoplasmic Reticulum Stress in HepG2 and Hep3B Cells

Figure 3

TNT triggered the mitochondrial dysfunction and apoptosis pathways. (A) TNT (0, 30, and 80 μM) caused a disruption of MMP in HepG2 cells, and TNT (0, 50, and 60 μM) caused a disruption of MMP in Hep3B cells after treatment for 24 h, as evidenced by an increased proportion of cells with green fluorescent light and a decrease proportion of cells with a higher red (JC-1 aggregates)/green (JC-1 monomers) ratio of JC-1 fluorescence. (B) TNT-induced mitochondrial dysfunction, as evidenced by Western blot analysis using antibodies against Bax, Bcl-2, and β-actin. (C) The cells were treated with different concentrations of TNT for 90 min in the presence or absence of 10 mM NAC, and the dissipation of MMP was measured. (the positive control: 100 μM H2O2)

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