Figure 3

Hepatic TonEBP, HMGB1, IL-6, and TNF-α expression in HFD/STZ-treated TonEBP^+/− mice. (a) Representative micrographs of TonEBP-immunostained liver sections; scale bar = 50 μm. Western blots and protein quantification of hepatic TonEBP (b), HMGB1 (c), IL-6 (d), and TNF-α (e). The mean values were obtained from at least three independent experiment (n = 6 mice per group). Cropped blots are displayed here and full-length blots are included in the Supplementary Information. Data are presented as the mean ± SEM. *P < 0.05 vs. control (CTL) normal diet-fed mice; ^† P < 0.05 vs. DM WT mice.