Figure 4

Hippocampal TonEBP expression in HFD/STZ-treated TonEBP^+/− mice. Western blots and protein quantification of total (a) and nuclear (b) TonEBP in the hippocampus. The mean values were obtained from three separate experiments (n = 6 mice per group). Cropped blots are displayed here and full-length blots are included in the Supplementary Information. Data are presented as the means ± SEM. *P < 0.05 vs. control (CTL) normal diet-fed mice; ^† P < 0.05 vs. DM WT mice. (c) Representative micrographs of TonEBP immunoreactivity in the hippocampus. TonEBP labeling resembles fine sand grains. The boxed areas of a’, b’, c’, and d’ are magnified on the right. Scale bar = 25 μm (inset, 5 μm). (d) The intensity of TonEBP immunoreactivity in the hippocampus was measured and presented as the fold change. Data are presented as the mean ± SEM. *P < 0.05 vs. control (CTL) normal diet-fed mice; ^† P < 0.05 vs. DM WT mice.