Figure 5

Effects of TonEBP haploinsufficiency on HMGB1, IL-6, and TNF-α expression in the hippocampus of HFD/STZ-treated mice. (a) Western blots and protein quantification of nuclear HMGB1 in the hippocampus. (b) Representative micrographs of HMGB1 immunoreactivity in the hippocampus. The boxed areas of a’, b’, c’, and d’ are magnified on the right. Scale bar = 25 μm (inset, 5 μm). (c) The intensity of HMGB1 immunoreactivity in the hippocampus was measured and presented as the fold change. (d) Western blots and protein quantification of hippocampal IL-6 and TNF-α. The mean values were obtained from three separate experiments (n = 6 mice per group). Cropped blots are displayed here and full-length blots are included in the Supplementary Information. Data are presented as the mean ± SEM. *P < 0.05 vs. control (CTL) normal diet-fed mice; ^† P < 0.05 vs. DM WT mice.