Figure 1

Generation of rMV-H5HA. (a) Structure of the full-genome cDNA of rMV-H5HA. (b) Comparison of growth curves for rMV-H5HA and the parental rMVs in the corresponding cell lines. Data are averages ± SEM (n = 3). Closed or open dots indicate rMV-H5HA or the parental virus, respectively. Expression of H5 HA protein in cells infected with rMV-H5HA was examined with immunofluorescent microscopy (c) and western blotting (d) Full-length blots of each protein are shown in Supplemental Figure S1) (c) H5 HA was detected with anti-H5 HA antibody (rabbit) and anti-rabbit IgG antibody conjugated with Alexa Fluor 568, and MV N was detected with an anti-CDV N monoclonal antibody (mouse) and anti-mouse IgG antibody conjugated with Alexa Fluor 488, respectively.