Figure 4
2D representation of predicted binding modes, mutagenesis data and residues involved in the binding of ggTas2r1 agonists. H-bond interactions are shown as magenta dashed arrows, salt bridges as blue-red lines, π−π interactions as green lines, cation−π interactions as red lines and hydrophobic interactions are represented as the distances between the closest heavy atoms of the ligand and the residue as orange dotted lines. Dose-response relations for the specified compounds in cells expressing point-mutated and native ggTas2r1 constructs: the y-axis shows the relative fluorescence changes (ΔF/F), and the x-axis the ligand concentration in μM. Green checks indicate residues involved in the binding, red crosses residues that are not involved in the binding, as deduced from experimental data: we consider “not involved in the binding” those residues that when mutated to alanine show a curve equal (or higher) than that of the wild-type, and “involved” the residues that mutated to alanine have a decreased response (with a change of at least ~0.1 ΔF/F).
