Table 2 Oligonucleotides used for site-directed mutagenesis of ggTas2r1.
Oligonucleotide | 5′ → 3′ Nucleotide sequence |
|---|---|
chTas2r1K86A_for | GTAACTTTCGC GACAGTTTTTATATTC |
chTas2r1K86A_rev | GAATATAAAAACTGTC GCGAAAGTTAC |
chTas2r1F89A_for | CAATGACAGTTGC TATATTCCTG |
chTas2r1F89A_rev | CAGGAATATA GCAACTGTCTTG |
chTas2r1N93A_for | GTTTTTATATTCCTGGC CTCTTATAGC |
chTas2r1N93A_rev | GCTATAAGAG GCCAGGAATATAAAAAC |
chTas2r1F181A_for | CAATTTGATTGC TTTGATCCTTC |
chTas2r1F181A_rev | GAAGGATCAAA GCAATCAAATTG |
chTas2r1L185A_for | GATCCTTGC CTGTAATGTTG |
chTas2r1L185A_rev | CAACATTACAG GCAAGGATC |
chTas2r1Y244A_for | CTCCTCCTGGC CATTACAAATTTTATC |
chTas2r1Y244A_rev | GATAAAATTTGTAATG GCCAGGAGGAG |
chTas2r1N247A_for | GTACATTACAGC TTTTATCGCTTTG |
chTas2r1N247A_rev | CAAAGCGATAAAA GCTGTAATGTAC |
chTas2r1L251A_for | CAAATTTTATCGCTGC GATTCTCATTTTATC |
chTas2r1L251A_rev | GATAAAATGAGAATC GCAGCGATAAAATTTG |
CMV_for | CGCAAATGGGCGGTAGGCGTG |
BGH_rev | TAGAAGGCACAGTCGAGG |
