Figure 3
From: Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA
Association of DNA with EXO surface is abolished by high salt concentration. Ciprofloxacin-exposed Jurkat cell exosomes (EXOs) were conjugated onto latex beads and washed using annexinV binding buffer supplemented with 0.1 M, 1 M or 2 M NaCl. All latex-bound EXO samples were re-suspended in annexinV binding buffer, and labeled with propidium iodide (PI), annexinV-FITC and an anti-CD63-PE antibody for flow cytometry. (a) Differences of geometric mean fluorescence values between EXOs washed in high salt concentration buffers and controls are shown. Plotted values are presented as the mean+/− S.D. (error bars) of three independent experiments. *P < 0.05, Friedman test, One-way ANOVA (b) Density plots show PI, annexinV-FITC and anti-CD63-PE fluorescence of latex-bound EXOs as a function of SSC parameter. Horizontal lines indicate fluorescence threshold of labeled EXO-free latex beads.
