Figure 6
Stingray venom induces IL-33 in heart and lung tissues. Venom (300 μg/ml) in 500 μl or sterile saline were injected i.p. into C57BL/6 WT or IL-33/citrine reporter (citR) mice. After 2, 4 or 8 h, mice were anesthetized with a ketamine (50 mg/ml)/xylasine (25 mg/ml) and perfused through the ascending aorta with saline followed by 4% paraformaldehyde. After perfusion, heart (A), lung (B) and lymph nodes (C) were removed and post fixed for two months. For cryosectioning, fixed organs were transferred to 40% sucrose in phosphate buffer, mounted in OCT compound (Neg-50, Richard Allan), sectioned at 15 μm on a cryotome (Cryostat HM 505E), and processed for immunofluorescence. All sections were stained with DAPI. IL-33-produncing cells (indicated by arrows) were imaged with an inverted fluorescence microscope (Olympus BX51 at 10x). Scale bar = 200 μm.
