Table 1 Primer sequences used for RT-PCR and qPCR.
From: Gene Location, Expression, and Function of FNDC5 in Meishan Pigs
Primer Name | Primer sequence |
|---|---|
Irisin-F | AGTCCCTCAGCCCCAGTGAA |
Irisin-R | CTCCTTCATGGTCACCTCGT |
Total FNDC5-F | ATGCACCCCGGGCCGCCC |
Total FNDC5-R | TGCTCTAACTGGATCGGAGG |
FNDC5-F | TGCAGGCCATCTCCATTCAG |
FNDC5-R | ATATTGGCGGCAGAAGAGGG |
UCP2-F | AGTGTGAGACCTGACGAAGC |
UCP2-R | CCTTTCTCCCTGGATCTGC |
UCP3-F | CAACAGGAAGTACAGCGGGA |
UCP3-R | CACCATCTCGGCACAGTTCA |
Cidea-F | GGGAGATAAGGGTCAGCGTG |
Cidea-R | AAGCAGAGATGAAGAGGAAGCA |
PGC-1α-F | CACCAGCCAACACTCAGCTA |
PGC-1α-R | GAGGTGCACTTGTCTCTGCT |
CD137-F | AAACAACCGTTTCTGAAGCCAG |
CD137-R | TCAAGAGAGTCCCAGCACCT |
AP2-F | GAAAGAAGTGGGAGTGGGCTT |
AP2-R | GGTGGTTGTCTTTCCATCCCA |
ADIPOQ-F | CGAGAAGCCTGGAGCACTAC |
ADIPOQ-R | CCTTCAACCCCAGTCACTCC |
PPARγ-F | GACCATTTCTGGGTCGCCT |
PPARγ-R | AAAGTTGGTGGGCCAAAACG |
TBP1-F | AACAGTTCAGTAGTTATGAGCCAGA |
TBP1-R | AGATGTTCTCAAACGCTTCG |
