Figure 7
Phylogenetic analysis of ubq genes and alignment of grass carp ubq-y gene with its female homolog. (a) Alignment of coding protein sequences between ubq-y gene and its female homolog in grass carp. Sequences were aligned with ClustalW2. The result showed widespread differences in amino acid sites, suggesting that ubq-y gene is a divergent paralog (amino acid identity of 75%). (b) Phylogenetic analysis of ubq genes. A Maximum Likelihood tree with Poisson correction was constructed using MEGA646. Bootstrap support values (10,000 replicates) are shown inside the tree. The tree is drawn to scale, with branch lengths measured using the number of substitutions per site (below the branches). All positions with gaps and missing data have been eliminated. Sequence accession numbers are shown in Supplementary Table S7. Phylogenetic analyses of ubq genes suggested that ubq-y gene diverged from the female homologous gene in certain fish species. Species information are: Sinocyclocheilus rhinocerous (Srh); Astyanax mexicanus (Ame); Cyprinodon variegatus (Cva); Oryzias latipes (Ola); Dictyostelium discoideum (Ddi); Homo sapiens (Hum); Danio rerio (Dre).
