Table 1 Efficiency of CRISPR-based editing of ADE2 in multiple isolates of C. parapsilosis.
From: Gene editing in clinical isolates of Candida parapsilosis using CRISPR/Cas9
Strain | sgRNA | % pink transformantsa | |||
|---|---|---|---|---|---|
−RTc | +RTc | ||||
Exp 1 | Exp 2 | Exp 1 | Exp 2 | ||
CLIB214d | sgADE2-B | 0% | 0% | 80% | 100% |
CLIB214d | sgADE2-D | 0% | 0% | 94% | 100% |
103d | sgADE2-B | 0% | 12% | 50% | 80% |
02-203d | sgADE2-B | 0% | 0% | 87% | 94% |
73/037 | sgADE2-B | 11% | 0% | 5% | 17% |
73/107d | sgADE2-B | 4% | 0%b | 4% | 16% |
74/046 | sgADE2-B | 0% | 0%b | 30% | 4% |
81/040 | sgADE2-B | 0% | 0% | 0% | 0% |
81/042d | sgADE2-B | 0% | 0%b | 8% | 14% |
81/253d | sgADE2-B | 0% | 1% | 12% | 35% |
90–137 | sgADE2-B | 20% | 10% | 37% | 67% |
CDC165d | sgADE2-B | 20% | 1% | 0% | 87% |
CDC167 | sgADE2-B | 0% | 1% | 15% | 14% |
CDC173 | sgADE2-B | 0% | 3% | 13% | 10% |
CDC177d | sgADE2-B | 4% | 0%b | 3% | 24% |
CDC179 | sgADE2-B | 0% | 11% | 35% | 25% |
CDC317 | sgADE2-B | 0% | 13% | 16% | 16% |
J931058 | sgADE2-B | 0% | 31% | 100% | 77% |
J931845 | sgADE2-B | 0% | 0%b | 28% | 13% |
J950218 | sgADE2-B | 11% | 1% | 14% | 7% |
J951066 | sgADE2-B | 0% | 14% | 11% | 85% |
J960578d | sgADE2-B | 0% | 4% | 25% | 13% |
J961250 | sgADE2-B | 0% | 0%b | 29% | 19% |
