Figure 1
From: HDAC3 inhibition ameliorates spinal cord injury by immunomodulation
Upregulation of HDAC3 in microglia/macrophages after SCI. (a) Representative sagittal immunohistochemistry images and quantification demonstrate upregulation of HDAC3 (green) at lesion center 7 days post injury (7 dpi) by T8 dorsal column transection (SCI-T). DAPI nuclear counterstaining highlights the increased cellularity at lesion core. Enlarged images of boxed areas are shown on the right. Quantification of the number of HDAC3+ cells at the injury site is shown on the right: *p = 0.015, parametric slit-plot ANOVA. 3 pairs of mice were examined, with 3 representative images quantified for each animal. (b) Representative immunohistochemistry images of HDAC3 at different time points after SCI-T as compared to sham. (c) Representative immunofluorescent images of co-immunolabeling show a large overlap of HDAC3+ cells (green) and CD11b+ or Iba1+ immune cells (red) at the injury site at 7 days after SCI-T. Enlarged images of boxed areas are shown on the right. (d) Immunohistochemistry images for CD11b (red) and different class I HDACs (green) at 7 days after SCI-T. No significant overlaps were detected between these HDACs and CD11b. Orientation: R: Rostral, C: Caudal, D: Dorsal, V: Ventral. Scale bars: 100 µm (a,b,c), 25 µm (d, enlarged images in a,c).
