Figure 2

Epithelial-mesenchyme transition (EMT) is induced in AFG1-induced lung adenocarcinoma. Paraffin sections and the total protein samples isolated from the lung-adenocarcinoma or control lung tissues were collected from our previous study. (a) Representative immunohistochemical staining of E-cadherin, vimentin, α-SMA and twist1 in AFG1-induced lung adenocarcinoma. (b) The expression of E-cadherin, vimentin, α-SMA and twist1 at protein level was shown by Western blot. Band intensity is coming from densitometry, and data was shown as mean ± SD (n = 3, *p < 0.05, compared to control group). (c) Representative immunohistochemical staining of MMP2 and MMP9 in AFG1-induced lung adenocarcinoma. (d) The expression of MMP2 and MMP9 at protein level was shown by Western blot. Band intensity is coming from densitometry, and data was shown as mean ± SD (n = 3, *p < 0.05, compared to control group).