Figure 2

(A) In vitro immunomodulatory effect of HD- and PID-MSCs on healthy donor (HD) peripheral blood mononuclear cells (PBMCs) in an allogeneic setting. The graph shows the percentage of residual proliferation of HD-PBMCs stimulated with phytohaemagglutinin (PHA) either in the absence (NO MSC) or in the presence of HD- (+HD-MSC), CGD- (+CGD-MSC), WAS- (+WAS-MSC), ADA- (+ADA-MSCs) and SCID-MSCs (+SCID-MSC). Each bar represents the percentage of residual proliferation of 10⁵ PBMCs, in the presence of two different MSC:PBMC ratios (MSC:PBMC ratio of 1:2 and 1:10), calculated by measuring 3H-thymidine incorporation after 72 hours co-culture. We referred to PBMC proliferation alone (in the absence of MSCs) as 100% and this percentage was used to normalize PBMC proliferation in the presence of MSCs. Mean ± SEM of multiple experiments performed at least twice on 9 HD-MSCs, 7 CGD-MSCs, 12 WAS-MSCs, 6 ADA-MSCs and 5 SCID-MSCs (each point being in triplicate) is reported. P values lower than 0.05 were considered to be statistically significant (*p < 0.05; ***p < 0.001). (B) Levels of anti-inflammatory and pro-inflammatory cytokines in supernatants of co-cultures of 3 HD-MSCs, 3 WAS-MSCs, 2 CGD-MSCs and 2 SCID-MSCs (RAG-1 deficiency) with PBMCs, after 72-hour incubation with PHA. Results are expressed as pg/ml of the mean ± SEM. P values lower than 0.05 were considered to be statistically significant (*p < 0.05).