Figure 1

(a) Biofilm-removal efficiency of a mixture consisting of betaine and erythritol. S. mutans biofilms were exposed to 5.6 mM betaine, 2.8 mM erythritol and a mixture thereof, respectively. (b) Effect of the molar ratio between betaine and erythritol on biofilm removal. Weight fractions of the mixtures of betaine and erythritol were 0.1% in all samples. (c) Effect of hydrogen bond donor (HBD) combined with betaine on biofilm-removal efficiency. Betaine was combined with glycerol, erythritol, xylitol, and sorbitol at molar ratios of 3:2, 2:1, 5:2, and 3:1, respectively. In all figures, each value and error bar indicates mean and standard deviation (SD), respectively (n = 3–6).