Figure 3
Mitochondrial reactive oxygen species and damage to macromolecules. (a) Mitochondrial superoxide anion level as indicated by dihydroethidium fluorescence (*=significantly increased compared to control; p ≤ 0.05). (b) Mitochondrial peroxyl levels as indicated by dihydrorhodamine fluorescence compared to control. (c) Membrane damage, indicated by percent lactate dehydrogenase release (*=significantly increased compared to control; #=significantly increased compared to other temperature groups; p ≤ 0.05). (d) Fold change mtDNA damage (*=significantly increased compared to control; p ≤ 0.05).
