Figure 4 | Scientific Reports

Figure 4

From: Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets

Figure 4

Cell morphology as shown by phase contrast (X200) micrographs. (a) Representative image of control. Gaps in the cell layer (arrows) were seen at 4 °C (b) and at 8 °C (c). Dead cells (arrowhead) were also present at 4 °C (b). The cell layer was similar to control at 12 °C (d). Large cells (arrows) were more frequent at 16 °C (e) and at 24 °C (f) Extended filopodia (arrowhead) were also seen at 24 °C (g) Cell size indicated by forward scatter by flow cytometry. (*=significantly increased compared to control; p ≤ 0.05).

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