Table 1 Percent Positive Cells (Immunocytochemistry).

From: Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets

 

Control

4 °C

8 °C

12 °C

16 °C

24 °C

ABCG2 [BXP21]

+++

++++

+++++*

+++++*

+++++*

++*

CK10 [polyclonal]

+

++

++

++

+++*

+++*

P63 [EPR5701]

+++++

++++*

++++*

+++++

+++++

++*

P63 [4A4]

+++++

++++*

++++*

+++++

+++++

++*

CK14 [LL002]

+++++

+++*

+++++*

+++++

+++++

++++*

Involucrin [SY5]

+

+

+

+

+

++*

C/EBPδ [polyclonal]

+

+

+

+

++*

+

PCNA [PC10]

+++

+++

+++

+++*

++++*

+++

Cleaved Caspase-3 [D175]

+

+

+

+

+

+

  1. *Significantly different compared to control (p ≤ 0.05). + = present. ++ = 20–39%. +++ = 40–59%. ++++ = 60–79%. +++++ = 80–100%.
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