Table 1 Summary on generation of CRISPR/Cas9 targeted lambs by injection of sgRNA/Cas9 mRNA into cytoplasm of zygotes.
From: Alteration of sheep coat color pattern by disruption of ASIP gene via CRISPR Cas9
Donor Ewe | Collected Embryos | Injected Embryo | Cleavage Ratio (%) | Embryos For ET | Surrogate Ewes | Conception Rate (%) (Pregnancies /Surrogates) | Lambs Born | Gene Modified Lambs | GM Ratio (%) (GM Lambs/ Lambs Born) |
|---|---|---|---|---|---|---|---|---|---|
14 | 113 | 105 | 87.62 (92/105) | 92 | 60 | 10 (6/60) | 6 | 5 | 83.33 (5/6) |
