Table 2 Genotyping of spontaneous mutations, sgRNA/Cas9 targeted modifications and phenotypes of five targeted lambs.
From: Alteration of sheep coat color pattern by disruption of ASIP gene via CRISPR Cas9
Lambs | Targeted Modifications | Frame Shift/In Frame | Premature Stop | Native Mutation | Phenotype | ||
|---|---|---|---|---|---|---|---|
GM081 | −4bp(TATC) | 3 N + 1 | N = 1 | trunkated | N53 Aa | D9N9N5N5 | badger face with black body coat |
−2bp(AT) | 3 N + 2 | N = 0 | trunkated | N64 Aa | |||
GM106 | −4bp(TATC) | 3 N + 1 | N = 1 | trunkated | N53Aa | N9N9D5N5 | badger face with black body coat and white dot on the hinder back |
−6bp(TCTATC) | 3 N | N = 2 | deletion | / | |||
+1 bp(A) | 3 N + 1 | N = 0 | trunkated | N65Aa | |||
GM110 | −4bp(TATC) | 3 N + 1 | N = 1 | trunkated | N53 Aa | N9N9N5N5 | Brown with badgerface and white color on the hinder tail and neck botom |
−27bp(TGG-AGTA) | 3 N | N = 9 | deletion | / | |||
GM109 | −2bp(AT) | 3 N + 2 | N = 0 | trunkated | N64 Aa | N9N9D5N5 | Black/Brown-White dot color(with white on the head, leg and neck) |
−4bp(TATC) | 3 N + 1 | N = 1 | trunkated | N53 Aa | |||
+3 bp(TTG) | 3 N | N = 1 | deletion | / | |||
GM105 | −2bp(TC) | 3 N + 2 | N = 0 | trunkated | N64 Aa | N9N9D5N5 | Black and white dotted |
+1 bp(A) | 3 N + 1 | N = 0 | trunkated | N65 Aa | |||
WT108 | / | / | / | / | Wild type | D9N9N5N5 | white |
