Figure 9
Inhibition of autophagy potentiated CoQ0-induced apoptosis in SKOV-3 cells. (A–D) Cells were pretreated with autophagy inhibitor (3-MA, 2 mM) for 1 h, and then incubated with CoQ0 (30 µM) for 24 h. (A) Induction of apoptosis (DNA fragmentation) was determined by TUNEL assay in the presence or absence of 3-MA. The green florescence indicates TUNEL-positive cells in microscopic fields (200 × magnification). (B) The fold of apoptotic cells was calculated by measuring the florescence intensity using commercially available software. (C) Cleavage of PARP with or without 3-MA pretreatment was determined by Western blot. (C Changes in protein intensities were quantified using commercially available software. Results expressed as mean ± SD of three independent assays (n = 3). Significant at ***p < 0.001 compared with untreated control, and significant at # p < 0.05; ### p < 0.001 compared with CoQ0 alone treated cells.
