Figure 6 | Scientific Reports

Figure 6

From: Identification of Novel Non-secosteroidal Vitamin D Receptor Agonists with Potent Cardioprotective Effects and devoid of Hypercalcemia

Figure 6

Effect of physiologic dose of VDR 4-1 (0.6 μμg/kg) on vitamin D deficient mice after TAC in vivo. (A–D) Morphologic and functional assessment of 1α-HO KO mice at 4 weeks after TAC. HW/BW ratio (A) LV mass (B) Fractional shortening (C) and ejection fraction (D) are displayed. *p < 0.05 vs Veh-Sham, **p < 0.05 vs Veh-TAC; N = 4–6. (E) Representative Anf, Bnp and μMhc mRNA expression. Only relevant PCR bands are shown. No alteration was done to the original gel images. 18 S was used as an internal control for RNA. (F–H) Quantification of Anf (F), Bnp (G), and μMhc (H) mRNA expression. D3 = 1,25-D3 (0.6 μg/kg), VDR = VDR 4-1 (0.6 μg/kg) 3 x/week i.p. Total volume injected = 50 μL/animal/injection. *p < 0.05 vs Veh-Sham, **p < 0.05 vs Veh-TAC; N = 4–6.

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