Figure 7
Effect of supraphysiologic dose of VDR 4-1 (3.0 μg/kg) on WT mice with normal vitamin D level mice after TAC in vivo. (A–D) Morphologic and functional assessment of 1α-HO KO mice at 4 weeks after TAC. HW/BW ratio (A), LV mass (B), Fractional shortening (C) and ejection fraction (D) are displayed. *p < 0.05 vs Veh-Sham, **p < 0.05 vs Veh-TAC; N = 4–6. (E) Representative Anf, Bnp and μMhc mRNA expression. Only relevant PCR bands are shown. No alteration was done to the original gel images. 18 S was used as an internal control for RNA. (F–H) Quantification of Anf (F), Bnp (G), and μMhc (H) mRNA expression. *p < 0.05 vs Veh-Sham, **p < 0.05 vs Veh-TAC; N = 4–6. (I) Serum Ca2+ level after long-term injections D3 = 1,25-D3 (3.0 μg/kg), VDR = VDR 4-1 (3.0 μg/kg) 3 x/week i.p. Total volume injected = 50 μL/animal/injection. *p < 0.05 vs Veh; N = 4–6. (J) Survival curve of mice after supraphysiologic dose of VDR 4-1.
